Research & Initiatives
High-throughput identification of Mycobacterium avium antibiotic tolerance genes
A genome-wide transposon screen was used to elucidate M. avium genes that play a role in the bacterium’s tolerance to first- and second-line antibiotics. A total of 193 unique M. avium mutants with significantly altered susceptibility to at least one of the four clinically used antibiotics tested, including two mutants (in DFS55_00905 and DFS55_12730) with panhypersusceptibility. The products of the antibiotic tolerance genes identified may represent novel targets for future drug development studies aimed at shortening the duration of therapy for MAC infections.
M. tuberculosis drives dysregulation of mitochondrial transfer RNA–derived fragments (tRFs) in macrophages.
Using a novel miRge2.0-based tRF-analysis tool, tRFcluster, to analyze independently generated and publicly available RNA-sequencing datasets following infection of macrophages with various bacteria, we found that M. tuberculosis infection uniquely increases the expression of mitochondria-derived tRFs rather than genomic-derived tRFs, suggesting an association with mitochondrial damage in Mtb infection.
Integrative Multi-Omics Reveals Serum Markers of Tuberculosis in Advanced HIV.
Plasma samples were analyzed by microRNA omics, metabolomics, and multiplex cytokine/chemokine arrays to identify an integrative signature associated with incident TB in cases vs. controls with advanced HIV infection.